WebSep 15, 2024 · Missing CitationIn the original article (Zhu et al., 2024) was not cited in the article. The citation has now been inserted in Materials and Methods, Plasmids Constructions, Paragraph 1 and Table 1 and should read:To generate the pYT3198 plasmid, two DNA fragments corresponding to approximately 2 kb of chu_3198 up- and … WebSep 6, 2024 · Flavobacterium johnsoniae SprB moves rapidly along the cell surface, resulting in gliding motility. SprB secretion requires the type IX secretion system (T9SS). …
Gene deletion strategy to examine the involvement of the two
Web(A) ompA promoter (pOmpA) was amplified from pYT313, PCR-mutagenized, and inserted into the pCP11 plasmid in front of the actA or actE gene, generating a series of plasmids … WebFeb 15, 2024 · Comprehension of the degradation of macroalgal polysaccharides suffers from the lack of genetic tools for model marine bacteria, despite their importance for … jennifer aniston inheritance from father
Transcriptome architecture and regulation at environmental …
WebNational Center for Biotechnology Information WebFeb 21, 2024 · The first step is to insert two DNA fragments upstream and downstream of porY into pYT313. Specific Content: For the past few weeks, we had conducted plasmid preparation from the Escherichia coli bacterium, otherwise known as E. coli, polymerase chain reaction (PCR), gel electrophoresis, purification, and digestion. WebSUPPORTING INFORMATION. Supplementary Methods2. M1. Construction of the bfpR, hfpR, hfpY and hfpRbfpR strains2. M2. Construction of pGi39 plasmid allowing chromosomal gene integra pa dept of revenue rent certificate