Webb9 okt. 2016 · Released AMC was measured using a Fluoroskan Ascent fluorometer (Thermo Electron) at 390 nm (excitation wavelength) and 460 nm (emission wavelength) for up to 120 min. The ATP-dependent 26S proteasome activities were measured in the presence of 0.1 mM ATP [ 40 ]. Webb23 nov. 2015 · Bacteria were grown in static conditions individually, all together, or in all combinations of eight species and exoproduct production was measured by colorimetric or fluorometric reagents to assess short chain and long chain lipases, choline-specific phospholipases C, and proteases.
MERS Main Protease (Mpro) Fluorescence Dose Response
Webb2 nov. 2007 · The aim of this work was to modify the method of Ladd and Buttler (1972), by substituting Tris–HCl buffer (pH 8.52) with demineralized water (DEMI H2O), in order to … WebbProtein quality control was evaluated assessing the efficiency of the Ubiquitin Proteasome System (UPS) and protein levels of autophagic markers. Changes in cellular bioenergetics were monitored by measuring oxygen consumption and … matthew johnson alcentra
Switch-on Fluorescence Analysis of Protease Activity with the ...
Webb9 okt. 2012 · We explored using AcGFP1 (a fluorescent mutant of the wild-type green fluorescent protein) as a donor and mCherry (a mutant of red fluorescent protein) as an … Webb1 maj 2013 · The assays described are based on measurement of a marker activity associated with survive cell number. These assays are used for measuring the results of cell proliferation, testing for cytotoxic effects of compounds, and required multiplexing as an internal control to determine viable cell number during other cell-based assays. Webb13 apr. 2024 · Heterogeneous protease biosensors show high sensitivity and selectivity but usually require the immobilization of peptide substrates on a solid interface. Such methods exhibit the disadvantages of complex immobilization steps and low enzymatic efficiency induced by steric hindrance. In this work, we proposed an immobilization-free strategy … matthew johnson columbia sc