Peasy e1
WebFeb 1, 2024 · The pEASY-E1-VpCTL and pEASY-E1-VpSABL plasmids were extracted and transformed into E. coli BL21 (DE3). Then SDS-polyacrylamide gel electrophoresis (SDS-PAGE) was used to test the positive transformants after induced by 1 mM IPTG for 4 h. WebSamples analyzed included low molecular weight protein markers (M, lane 1), un-induced expression of pEASY-E1/ZmCAT in total BL21 (DE3) cell lysates (U, lane 2), 1 mM IPTG induced expression of ...
Peasy e1
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WebFeb 10, 2015 · The pEASY-E1 Expression Kit was purchased from Transgen Biotech. Recombinant DNA techniques were performed according to the manufacturer’s … WebOct 29, 2024 · The 2952-bp styS gene was amplified by PCR from the P. putida B3 genome using primers F/R-pEASY-styS ( Table 2 ), and the recombined plasmid pEASY-E1-styS was then constructed. Subsequently, the pEASY-E1-styS vector was introduced into E. coli BL21 (DE3), and the StyS protein was obtained by adopting the same method used for the StyR …
WebJul 26, 2024 · The PCR products were cloned into pEASY-E1 expression vectors (Transgen, Beijing, China). BL21 (DE3) competent cells were transformed with the pEASY-E1 plasmids containing the HMW-GS genes and then inoculated into 10 mL LB, with shaking at 37 {^ {\circ }}\hbox {C} under 200 rpm. WebAug 1, 2024 · The PCR fragment containing the r-agkihpin gene was purified and cloned into a pEasy-E1 vector to obtain the expression plasmid pEasy-agkihpin which included a His 6-tag (Fig. 1). The results of gene sequencing showed that the sequence of PCR product of pEasy-agkihpin was identical to the . Discussion. Agkihpin, ...
WebJul 1, 2012 · Amplified PCR products were digested with the respective restriction enzymes and cloned in frame with pEasy-E1 vector (TransGen, Beijing, China), resulting in pEasy-E1-CsTS. After confirming the cloned fragments by DNA sequencing, pEasy-E1-CsTS was transformed into the Rosseta good (DE3) cells and the recombinant CsTS protein … WebJul 7, 2015 · The production process is characterized by including that plasmids of tafazzin full-length genes are used as a template, the tafazzin genes are amplified through PCR …
WebJan 8, 2016 · The LB medium with pEASY-EaCAT and pEASY-SoCAT was induced for 2 and 4 h at 37 °C, and 1.0 mL medium was collected at each time point. The LB medium with empty pEASY-E1 was induced with IPTG for 6 h, and 1.0 mL corresponding medium was collected. The medium collected was used for the SDS-PAGE with 25 μL loading sample.
Weberichiacoli and pEASY-E1 (T vector) were purchased from Transgen(Beijing,China).PronasewasfromM&CGeneTech-nology (Beijing, China). Cell Lines—Human lung adenocarcinoma cell line A549, human hepatocellular carcinoma cell line HepG2, human mammary epithelial cell line MCF10A, and human breast car-cinoma … chiefs nfl stream redditWebJan 10, 2010 · The cell suspension of the transformant E. coli BL21 (DE3) pLysS/pEASY-E1/aiiA induced by IPTG was analyzed with SDS–PAGE. The apparent bands with molecular weight of about 28 kDa were detected on the gel in the cell lysates of E. coli BL21(DE3)/pEASY-E1/aiiA after incubation for 1–4 h induced with IPTG, but not in those … chiefs niners predictionWebFeb 13, 2024 · Previous studies demonstrated that baijiu yeasts could generate 2-furfurylthiol using furfural and l-cysteine as precursors and that the Saccharomyces cerevisiae genes STR3 and CYS3 are closely... chiefs nfl roster 2018