WebMay 12, 2024 · Given the large number of potential CRISPR arrays in the genome, the approach could in principle be scaled up to allow whole-organism lineage tracing. This … WebThe CRISPR-based tools and methods developed here could inform the use of CRISPR/Cas9 in mammalian cell lines, accelerate mammalian cell line engineering, and support advanced recombinant protein production applications. ... which share a common lineage (Feng et al., 2024; Shaner et al., 2004 ... Multiple SSI formats using Cas9 …
Large-scale reconstruction of cell lineages using single-cell …
WebJun 15, 2016 · Initially assessed in a human embryonic kidney cell line, this strategy took advantage of combinatorial and cumulative CRISPR/Cas9-based genome editing to introduce mutations in a DNA barcode. In this case, the barcode consisted of a contiguous artificial array of CRISPR/Cas9 target sites that can be easily read through single cell … WebJan 28, 2016 · CRISPR makes it easy to target multiple loci - a concept called multiplexing. Since CRISPR is such a robust system, editing or labeling efficiency doesn’t usually change when you add multiple … flying coffin shirt
Single-cell Lineage Tracing by Integrating CRISPR-Cas9
WebApr 5, 2024 · Upon CRISPR editing, we performed targeted amplicon sequencing using Illumina MiSeq on the edited cell pools to quantify the induced indels across multiple observation timepoints. For exon targeting (g 1 ) in cell line HAL-01, the induced indels will lead to frameshift if the length is not 3n (3, 6, 9, etc.), which can be analyzed by CRIS.py ... WebJul 16, 2024 · CRISPR-based diagnostics have been used for a wide range of biomedical applications, and in particular for the sensing of nucleic-acid-based biomarkers of infectious and non-infectious diseases ... WebMay 12, 2024 · In both mouse and zebrafish, we identify the highest quality compact arrays as judged by equal base composition, 5' G sequence, minimal likelihood of residing in the functional genome, minimal off targets and ease of amplification. We validate multiple high-quality endogenous CRISPR/Cas9 arrays, demonstrating their utility for lineage tracing. flying coffin